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KMID : 1161420110140070761
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Journal of Medicinal Food
2011 Volume.14 No. 7 p.761 ~ p.766
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Photoprotective Effects of Two Natural Products on Ultraviolet B?Induced Oxidative Stress and Apoptosis in SKH-1 Mouse Skin
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Filip Adriana
Daicovici Doina
Clichici Simona
Mocan Teodora
Muresan Adriana
Postescu Ion Dan
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Abstract
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Solar ultraviolet radiation (UV) is the major cause of nonmelanoma skin cancer in humans. Photochemoprevention with natural products represents a simple but very effective strategy for the management of cutaneous neoplasia. We studied the photoprotective activity of Calluna vulgaris and red grape seed (Vitis vinifera L, Burgund Mare variety [BM]) extracts in vivo in an SKH-1 hairless mice skin model. Fifty 8-week-old female SKH-1 hairless mice were randomly divided into 5 groups (n?=?10 each): controls, UVB-irradiated, C. vulgaris plus UVB?irradiated, BM plus UVB?irradiated, and epigallocatechin gallate (EGCG) plus UVB?irradiated. A dose of 4?mg/mouse per cm2 of skin area for both extracts was topically applied to the mice 30 minutes before a single-dose (240?mJ/cm2) UVB exposure. EGCG dissolved in phosphate-buffered saline (pH 6.6; 0.067?M) was administered at 2?mg/mouse per cm2. Glutathione peroxidase and catalase activities, reduced glutathione (GSH), malondialdehyde, nitric oxide, and caspase 3 activity were determined in skin homogenates 24 hours after irradiation. A single dose of UVB increased GSH levels and glutathione peroxidase activity in the exposed skin. C. vulgaris and BM pretreatment significantly decreased GSH formation and glutathione peroxidase activity (P?
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KEYWORD
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grape seed, oxidative stress, apoptosis, chemoprevention, nitric oxide
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